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In diet-induced obesity animal models, NNMT inhibition demonstrated metabolic changes including alterations in adipocyte lipid content without affecting food intake patterns, coupled with significant reductions in white adipose tissue mass in treated mice

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Samples were quantified using densitometry by quantifying the volume of the primary SUMOylated TDP-43 band at ~ 98 kDa, standardizing to the volume of the immunoprecipitated TDP-43-GFP signal at ~ 70 kDa, and normalizing to the stressed positive control sample without sgRNA on each blot

1984;36(2):25S-33S 30