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TLR-targeted therapy: Challenges and future perspectives Simultaneous activation of anti-/pro-tumor mechanisms As TLRs regulate a wide range of functions, initiating signaling pathways that may lead to both anti-tumor and pro-tumor effects, there is a concern regarding whether the application of TLR agonists will promote tumor growth or whether the anti-tumor effect will be counteracted by the pro-tumor effect they simultaneously initiated

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The strategies have focused on the application of small-molecule inhibitors, natural compounds, nanocarrier-mediated co-delivery of DOX with autophagy modulators and the development of combination therapeites providing the crosstalk of autophagy and cell death mechanisms in DOX resistance