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For experiments, the synchronized L1 larvae were placed on the new NGM plates containing OP50 and incubated at 20C and allowed to grow to L3 larvae

After washing another time, the cells were dehydrated in a graded series of alcohol (30%, 50%, 70%, 80%, 90%, 95%, and 100%) before being embedded in EMBed 812 (SPI, United States), and ultrathin sections (6080 nm) were prepared using a Leica ultramicrotome (Leica Microsystems, United States) and fished out onto the 150-mesh cuprum grids with a formvar film

These findings not only provide valuable insights into the molecular basis of exercise-induced protection against ferroptosis-mediated cellular damage but also offer novel perspectives for future investigations into exercise-based interventions for NAFLD management

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