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Type of sampling and reasons for selection Whole blood, serum harvested from blood samples, and tissue homogenates were selected for investigation in this study

After cooling and PBS rinsing, sections were blocked with 5% BSA (30 min, RT), incubated with primary antibodies (4 C overnight), rewarmed, and washed

Scleratogenous referred pain60 arises from irritation of the somatic tissues surrounding deep jointsespecially joint capsules, intervertebral disks, and articular ligaments

The physiological functions regulated by nitric oxide include metabolism, muscle contractility, neurological functions, the immune system, and others