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To Eliminate non-specific adsorbents caused by hydrophobic adsorption: Wash with 3-4 column bed volumes of 70% ethanol or 2 column bed volumes of 1% Triton X-100, followed immediately by 5 column bed volumes of PBS, pH 7.4.

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platensis cells were high-pressure frozen using a Leica EM PACT2 instrument (Leica Microsystems, Buffalo Grove, IL), cryo-substituted for 80 h at 87 C in acetone containing 2% osmium tetroxide, and, after slowly warming to RT, embedded in Spurrs resin