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The mouse tissues were transferred into homogenizer tubes and homogenized with H 2 O at a ratio of 200 L H 2 O per 20 mg tissue and ceramic beads using a homogenizer (JXFSTPRP-CL, Shanghai Jingxin Experimental Technology) at the low-temperature condition

These findings demonstrate that crude HEM and its active substances (i.e., HeA, HeC, and HeS) can regulate the function of CNS neurons, astrocytes and microglia via either NGF activity or anti-inflammatory effects

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Notably, exercise positively influences insulin interactions, enhancing its efficiency in transporting glucose into muscle cells