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Glycine is synthesized primarily through the enzymatic conversion of serine-by-serine hydroxymethyltransferase (SHMT), which operates in both the cytosol and mitochondria, catalyzing the reversible transfer of a hydroxymethyl group from serine to tetrahydrofolate (THF), producing glycine and 5,10-methylene-THF

Horvth E, Bela K, Gall , Riyazuddin R, Csomor G, Csenki D et al (2020) Compensation of mutation in Arabidopsis glutathione transferase ( AtGSTU ) genes under control or salt stress conditions

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A control was also performed simultaneously, where the activity was halted immediately at zero time