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Conversely, GADD45 overexpression in HepG2 cells reversed the PA-induced suppression of SIRT1 and upregulation of lipogenic genes

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MRM multiple reaction monitoring the 1-MNA NNMT reaction product was quantified from peak area ratios using AB Sciex Analyst and MultiQuant 2.1 software and the parent precursor and Q3 masses set to m/z 137.1 and 94.1, respectively