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Description

The quantitative analysis of the ALP and Alizarin Red staining area is shown in Fig

10.1038/nm.3193 71 DengP

Briefly, 10 g of recombinant protein were added to 1 ml of Triton X-114 (0.5%, 1%, 2%, or 4% in PBS, pH 7.4) in an Eppendorf tube (final concentration 0.3 M)