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HULEC-5a (passage 23) were maintained on pre-coated (fibronectin and collagen) T75 flasks using M199 medium supplemented with 15% FBS, 2 mM Glutamax I (Sigma, Germany: 35050061), 25 g/ml sodium heparin (Sigma, Germany

brand, containing a nicotine strength of 1.8%, which is a popular strength consumed by experienced EC users [10] and is close to the maximum level set by the European Commission regulatory proposal [11] ( The EC devices used in this study were rechargeable cigalike devices consisting of a cartomizer, which combines a disposable cartridge (which holds a liquid solution) and a built-in atomizer, and a rechargeable battery with a glowing red light-emitting diode (LED) tip on the end that lights up with each puff and serves as an indicator of use

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Just like his onscreen fasting, Tommy's cigarette routine is a constant throughout the series