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Exp Ther Med 2:481486 Sun RC, Board PG, Blackburn AC (2011) Targeting metabolism with arsenic trioxide and dichloroacetate in breast cancer cells

Tissue collection Mice from each treatment group (46 animals) were euthanized through an overdose of sodium pentobarbital (300 mg/kg, Euthasol) via IP injection, followed by transcardial perfusion with 0.1 M PBS, then 4% paraformaldehyde (PFA) for tissue fixation

This suggests a possible positive selection in neoplastic cells for mutated mitochondrial genes, in keeping with observations that an aberrant mitochondrial respiration confers adaptive advantages to neurofibroma cells

GSSG was reduced to GSH by the action of GR and NADPH