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Bibcode:1964Natur.203.1068H

This results not just in dehydration, but an electrolyte imbalance

10.1186/s12974-017-0839-0 [DOI] [PMC free article] [PubMed] [Google Scholar] Cappelletti S., Piacentino D., Sani G., Aromatario M

The following day, cells were rinsed with PBS and incubated with 1:300 secondary Goat anti-Mouse IgG Alexa 488 (Thermo Fisher Scientific) in 16% (v/v) goat serum with 0.3% (v/v) Triton X-100 for 1 hour at room temperature, followed by PBS washing