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The target gene expression was normalized to those of -actin, while the relative miRNA levels were normalized to the U6 or cel-miR-39 in sEV and calculated as 2 (CT)

In-depth study of mechanisms of action revealed that the nanoreactors could catalyze the oxidation of GSH into GSSG

Phylogenetic analysis Eukaryote orthologs of ADH5 were obtained from NCBI, CLUSTAL at phylogeny.fr was used to align the sequences, and TreeDyn at phylogeny.fr for tree generation 66