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It is a metabolic stressor one that actively worsens insulin sensitivity, elevates cortisol, disrupts GH secretion, upregulates hunger hormones, and promotes visceral fat accumulation

Means were calculated across replicate cultures: glpQ 1 = 6 replicates, parent = 8 replicates

After further culturing for 24 h, each well was washed, 1 mg mL 1 MTT solution (100 L) was added, and the cells were re-incubated for 4 h in the dark

Additionally, cholesterol molecules occupy the grooves between TM5:TM7 and TM2:TM7 which have been demonstrated to influence the conformation of DAT during the transport cycle of the substrate (Hong and Amara, 2010