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Immunofluorescence staining of oocytes After a brief rinse with PBS/0.05% polyvinylpyrrolidone (to prevent adhesion), the oocytes were permeabilized with 0.5% Triton X-100/PHEM for 5 min, fixed in 3.7% PFA/PHEM (18.14 g PIPES, 6.5 g HEPES, 3.8 g EGTA, 0.99 g MgSO4, pH 7.0 w/KOH) for 20 min, and washed three times (10 min each) with PBS/polyvinylpyrrolidone

Lonial S, Jagannath S, Martin T, Wang M , Singhal S, Trudel S, Le M, Kirk C, Arastu-Kapur S, Vij R, Siegel D

It includes waterborne parasites, industrial chemicals, and others

BAs are not only essential for fat digestion but they also regulate gene expression and promote inflammation