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The formation of TLS was suppressed in the kidneys of sulfasalazine-treated mice on day 21, although still observed (Figure 5, A and B, Supplemental Figure 12)

2448 h post-transfection, the transfected cells were identified and collected by fluorescence-activated cell sorting based on GFP fluorescence using a BD FACSAria II sorter (BD Biosciences, San Jose, CA) equipped with a GFP filter (530/30 nm)