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Mice were then transcardially perfused with ice-cold PBS (Biological Industries) before spleens, deep cervical lymph nodes, Peyers patches, and mesenteric lymph nodes were excised and mashed with the plunger of a syringe against a 100 m pore size cell strainer (Corning)

Step 4: Choose Syringe Type Select the syringe you use so the calculator can show the most practical measurement format

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