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Anti-phospho-Tyr99 (sc-7020, 1:1000) from Santa Cruz

et al., 2021), ALS (Yu et al., 2020), multiple sclerosis (Woo et al., 2024), and neovascular age-related macular degeneration (Tanaka et al., 2024)

The retarded leaching of nanoclusters can be attributed to their large size in comparison with molecular dyes and it is a clear advantage for imaging purposes as dye leaching decreases the fluorescence brightness of NPs and increases the background signal resulting in unfavorable imaging inefficiency/artifacts 50