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Transfected HEK293 cells expressing MAST4 WT -mCherry and FLAG-Tctex-1 WT were rinsed twice with cold STE buffer (50 mM TrisHCl, 150 mM NaCl, and 2 mM EDTA, pH 7.4) and then lysed in STET buffer (STE buffer supplemented with 1% Triton X-100, protease inhibitor cocktail, 1 mM phenylmethylsulfonyl fluoride, and phosphatase inhibitor cocktail) at 4C for 30 min