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Detection of TBTV, TVDV, TBTVsatRNA and TVDVaRNA infections in the field-collected samples To determine the occurrence of TBTV, TVDV, TBTVsatRNA and TVDVaRNA in the field samples, virus specific primers (Supplementary Table 2) were used and the four viruses were simultaneously detected in the field-collected samples through multiplex RT-PCR as previously described 15

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