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doi: 10.2147/DDDT.S493923

This study followed a cohort of more than 800 participants over a 20-year period and found that individuals with higher dietary spermidine intake had a significantly lower risk of death from all causes, including cardiovascular disease and cancer

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1 g PCR-amplified biotinylated CGI Lhx6 was incubated with avidin beads for 2 hours at 4 C and subjected to 3 wash with the wash buffer (50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 0.1% NP40, 2 mM DTT, and 10% Glycerol) to remove the unbound DNA