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As our in vivo scRNA-seq data did not wholly capture the heterogeneity reflective of this progression, we studied the process by culturing basal tracheal epithelial cells at airliquid interface (ALI) and harvesting replicate cultures at 20 timepoints across mucociliary differentiation for scRNA-seq analysis (Fig

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In their place, he hired session pros like Stax Records veteran bassist Donald "Duck" Dunn and Muscle Shoals drummer Roger Hawkins, also bringing in guest guitarist Ry Cooder