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After a single hydroxocobalamin injection, therapeutic levels can be maintained for approximately 8 to 12 weeks in most patients, which aligns with the standard maintenance schedule of injections every 2-3 months recommended in the British National Formulary (BNF)

Subcutaneous (SubQ) injection is the better route for most 5-Amino-1MQ protocols due to consistent absorption kinetics, lower tissue trauma across repeated dosing, and easier self-administration without specialized technique

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