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Description

10 M)-treated ARPE-19 and H1299 cells

The dosage charts in this guide are derived from Phase 2 and Phase 3 clinical trial protocols, not from approved prescribing information

Bacterial pellets were resuspended and sonicated in lysis buffer (20 mM HEPES-KOH pH 7.5, 400 mM NaCl, 30 mM imidazole, 10% glycerol and 1 mM dithiothreitol) and purified using Ni-NTA resin (Qiagen, 30230)