Bulletin of Experimental Biology and Medicine, 158 , 726-728
Disclosures: Jessica Sosio: Nothing to Disclose, Karla Geisse: Nothing to Disclose, Lindsey Valdiviez: Nothing to Disclose, Roudi Bachar: Nothing to Disclose, Adrian Singson: Nothing to Disclose, Oluwagbenga Serrano: MERCK: Stock - publicly traded company 2229 ALFAPUMP SYSTEM IN REFRACTORY ASCITES: RESULTS FROM THE POSEIDON STUDY AT 24 MONTHS Hugo Vargas 1 Deepika Devuni 2 Mangesh Pagadala 3 Angela Cheung 4 Sumeet Asrani 5 Gijs Klarenbeek 6 Jeroen Capel 6 Jasmohan Bajaj 7 K Rajender Reddy 8 Patrick Kamath 9 Florence Wong 10 , 1 Mayo Clinic Arizona, 2 University of Massachusetts, 3 Methodist Transplant Center, Dallas, 4 The Ottawa Hospital, 5 Liver Consultants of Texas, 6 Sequana Medical, 7 Virginia Commonwealth University, 8 University of Pennsylvania, 9 Mayo Clinic Rochester, 10 University of Toronto Background: The standard of care for refractory ascites (RA) in cirrhosis is repeated large volume paracentesis (LVP)
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After 1 week to 10 days, the organoids were removed from the Matrigel or BME2, mechanically dissociated into small fragments using TrypLE Express (Gibco) and transferred to fresh Matrigel or BME2
