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Daily Dose: 2.67 mg (equivalent to 10 units / 0.10 mL), maintained throughout the full cycle Frequency: Administer once daily via subcutaneous injection, ideally at the same time each day Cycle Duration: 4-16 weeks of continuous use, followed by a 24 week break to assess response and support receptor recovery Injection Volume: Approximately 0.10 mL per dose, well within typical comfort limits for subcutaneous delivery (generally under 1.01.5 mL) Syringe Guidance: A 0.3 mL (30-unit) U-100 insulin syringe is recommended for improved accuracy when measuring small volumes Storage Instructions Proper storage is essential to maintain peptide stability and integrity

Test compounds (final concentration 10 M) were added with a 50-nl pin tool and the plates were incubated for 30 min at 20 C

doi: 10.1039/C4CS00373J 94

doi: 10.1002/fsn3.2921 139 PurushothamanJRRizwanullahMPurushothamanR