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Effect of glutathione depletion on Glutathione S-Transferase (GST) activity

Inhibition of GLS was found to promote apoptosis and suppress the growth and proliferation of AML cells [61, 62]

EW macrophages have a decreased ability to adhere when compared with the control group, but glutamine supplementation at 1 and 2 mM were capable of reversing this effect

For IF staining, fixed with 4% PFA for 15 min, permeabilized with 0.3% Triton X-100 in PBS for 10 min, and blocked with 5% normal donkey serum for 1 h at room temperature (RT)