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At specific time points, the supernatant of the samples was taken from one of the microtubes after centrifuging at 21,000 g for 60 min, and the concentration of siRNA in the supernatant was measured to determine the amount of released siRNA at each time point during 7 days after synthesis

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This finding further supports the conclusion that, under the current study conditions, hydrogen inhalation has not yet demonstrated a clear effect on symptom improvement (Yoritaka et al., 2021)