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Other potential factors impacting T4 to T3 conversion include: Aging Alcohol [7] Chemotherapy [8] Cigarettes [9] Deiodinase gene variation Diabetes [10] Fasting and calorie restriction [11] Growth hormone deficiency [12] Heavy metals [13] Iodine excess [14] Kidney and liver disease [15] Low progesterone [16] Medications Obesity [17] Pesticides [18] Radiation [19] Soy [20] Surgery [21] Furthermore, in recent years, pharmacogenomics (the study of how a persons genes impact their response to medications) has helped us determine which patients may best benefit from particular medications due to their unique genes

This separation protocol was first described for the isolation of DC from mouse Peyers patches, lymph nodes and spleen and for the isolation of Langerhans cells from skin [10-12]

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