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Salt stress and silicon influence plant physiology

Methylcobalamin is the active, methylated form

Assay Principle The low concentration of GSSG (high GSH/GSSG ratio) in tissues coupled with the need to prevent GSH oxidation during sample preparation, are important considerations for the accurate measurement of GSSG and GSH/GSSG ratios

Aliquots of 300 ul of the supernatant were transferred to fresh tubes for further analysis, while the remaining supernatant fluid was discarded and the protein pellet resuspended in 200 ul of 1 N NaOH and analyzed for protein quantification using the BioRad DC Assay with BSA as a standard