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the backbone moieties may carry groups that exhibit UV absorption at wavelengths where other degradation products do not exhibit UV absorption

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The supernatant (40 l) was added to a 160-l reaction mixture containing 143 mM of Na phosphate buffer (pH 7.5), 6.3 mM of EDTA, 0.22 mM of NADPH, and 20 l of 6 mM of 5,5-dithiobis-2-nitrobenzoic acid