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Research demonstrates that copper-binding peptides including GHK-Cu suppress the NF-B pathway through direct molecular interactions, with nuclear translocation of NF-B p65 decreased by approximately 30% upon treatment and molecular modeling showing specific peptide sequences exhibit high binding affinity to NF-B p65 (up to -8.8 kcal/mol)

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Furthermore, additional possible antiviral modes of action include inhibiting viral multiplication, reducing viral mRNA expression levels, producing changes in viral genome conformation (Memariani et al., 2020), inhibiting the viruss cytotoxic properties, and inhibiting virally mediated cell fusion (Mohanty and Murhekar, 2023), and inhibit viral bundling (Peng et al., 2023)

In EBOV, removal of the insertion element from the L protein abolishes its transcription activity 8