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The serial sections were sequentially mounted on microscopic slides and the motor neurons labeled by TB and/or FG were visualized and all the sections which showed tracer labeling were photomicrographed with an SP2 laser scanning confocal microscope (Leica Microsystems GmbH, Heidelberg, Germany)

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The ANP class of MAPKKKs from Arabidopsis can be induced specifically by H2O2, and this then activates a specific class of stress-induced MAPKs (Kovtun et al., 2000)