The blots were incubated with the following primary antibodies: GAPDH (1:10,000, Proteintech, 60,0041-Ig), HK2 (1:1000, Abcam, ab209847), TH (1:5000, Abcam, ab137869), LDHA (1:1000, Cell Signaling Technology, 3582S), Bax (1:1000, Abcam, ab32503), Bcl2 (1:1000, Abcam, ab32124), AMPK1/2 (H 300) (1:500, Santa Cruz Biotechnology, sc-25,792), phospho-AMPK1/2 (Thr 183/172) (1:500, Santa Cruz Biotechnology, sc-101,630), phospho-Akt (Thr308) (1:1000, Cell Signaling Technology, 4056S), Akt (1:1000, Cell Signaling Technology, 9272S), phospho-mTOR (Ser2448) (1:1,000, Cell Signaling Technology, 5336S), mTOR (7C10) (1:1000, Cell Signaling Technology, 2983S), -Synuclein (1:1000, Cell Signaling Technology, 4179S), BDNF (1:1000, Abcam, ab108319), PGC-1 (1:100, Abcam, ab191838), PDK1 (1:2000, Abcam, ab202468), PKM2 (1:1000, Cell Signaling Technology, 4053), GPX4 (1:1000, Abcam, ab125066), UCP2 (1:1000, Abcam, 89326), ATP5A (1:1000, Cell Signaling Technology, 18023), UQCRC2 (1:1000, Cell Signaling Technology, 99258), MTCO1 (1:1000, Abcam, ab14705), SIRT1 (1:1000, Abcam, ab110304), HIF-1 (1:1000, Abcam, ab308433), NRF1 (1:1000, Abcam, ab175932), and NRF2 (1:1000, Abcam, ab207223)
According to the American Heart Association, the leanest cuts of beef include sirloin, chuck, loin, or round
Specific targeting of deubiquitylating enzymes or E3 ubiquitin ligase enzymes that are involved in well-characterized disease processes will enable the development of specific new treatment options in renal disease
Morning or afternoon
