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Biochimica biophysica acta Mol

As depicted in the blot above, a household gene, such as -actin or gap GH, is used as a control since they are typically expressed uniformly across the sample

Indeed, the determination of the perinuclear mitochondrial CMFDA staining after co-localization with mitotracker red (as described previously [24]) (figure 4C) in 3T3 fibroblasts at 24 hours of culture show that BSO treated cells maintained a high mitochondrial CMFDA staining but cytosolic fluorescence was indeed decreased by BSO