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The AAVs used in this study were shown as follows: pAAv-CMV-MCS-EF1a-EGFP-tWAP, pAAv-CMV-Ndufa1-3 FLAG- EF1a-EGFP-tWAP were from Obio Technology (Shanghai, China)

Most multicellular and some unicellular eukaryotes, but not some insects such as Drosophila species, possess a mitochondrial proton-motive force-driven transmembrane transhydrogenase homologous to human NNT (Zhang et al., 2017), while bacteria can possess a transmembrane transhydrogenase, a soluble transhydrogenase, or rarely both (Sauer et al., 2004)

PT-141 works differently