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Proteins were detected using chemiluminescent substrate (Amersham ECL-Prime), pictures of membranes were acquired with a G:Box (Syngene), and integrated intensities quantified with ImageJ available at CK1D1 and CK1D2 antibodies were raised by the Yanaihara Research Institute using the peptides KLH-Cys-IPGRVASSGLQSVVHR for CK1D1 and KLH-Cys-NSIPFEHHG for CK1D2, corresponding to the specific C-terminal tail of each isoform