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Plasmid construction, growth and purification The pCTV plasmid containing the gene sequence encoding S protein, used in the immunization of experimental animals, was developed at the Centro de Tecnologias de Vacinas (UFMG)

Armogida et al

The genes encoding the three APR isoforms, in particular APR1 (At4g04610) and APR3 (At4g21990), were induced at all three time points, with a maximum after 1 h of allyl-ITC treatment