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Ca 2+ acts as a pivotal regulator in the excitation-contraction coupling of cardiomyocytes and serves as an essential second messenger in the control of metabolic activities, apoptotic pathways, and transcriptional regulation[125]

In addition, the GPx1 regulation of local H 2 O 2 level realized by binding TRAF2 is distinct from the regulatory action of Prx1, which forms a mixed disulfide with ASK1 to relay the oxidation signal 47

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Future efforts should focus on improving sensitivity, standardization, and accessibility, while leveraging single-cell and spatial omics to capture intratumoral heterogeneity and accelerating the translation of these methodologies into clinical practice