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Samples were then incubated at 95 C for 10 min, before storage at 80 C for subsequent immunoblotting analysis

Moreover, pharmacological doses of ZnO may interact negatively with other nutrients (38), promote the acquisition of antibiotic multi-resistant genes in zoonotic pathogens such as enterotoxigenic Escherichia coli (ETEC) (39), contributing to the emergence of antimicrobial resistances (AMR) and, finally, exacerbating public health concerns (33, 40, 41)

When the oxygen supply is insufficient, a large amount of pyruvate is produced by anaerobic glycolysis and converted to lactic acid to maintain the energy supply

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