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2.4 Antioxidant Enzymes Extraction and Assay Fresh leaf samples (0.5 g) were ground with 5 mL of ice-cold 100 mM phosphate buffer (pH 7.4) containing 1% polyvinyl pyrrolidine and 1 mM EDTA, then centrifuged at 15,000 g for 10 min at 25 C

Participants received 30 or 60 mg/day of Q10 for 100 days, and serum samples were analyzed for Q10 concentrations, malondialdehyde, total antioxidant status, SOD, and glutathione peroxidase, using ELISA assays

References Duniewska J., Sarnowska A., Beresewicz M., Johnson I., Srai S.K.S., Ramesh B., et al

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