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Subsequent work by Sun and colleagues in the APP/PS1 mouse model identified downstream PI3K/AKT pathway activation as a major component of the mechanism, with the PI3K inhibitor wortmannin reversing Dihexas anti-inflammatory and anti-apoptotic effects [5]

siRNAs were from GE Life Sciences/Dharmacon: Non-targeting Pool (D-001810-10-50), Myc (L-040813-00-0010), Atf4 (mouse, L-042737-01-0020), Rheb (L-057044-00-0020), RhebL1 (L-056074-01-0020), Tsc2 (L-047050-00-0020), C/ebp (L-040561-00-0005), C/ebp (L-043110-00-0005), C/ebp (L-060294-01-0005), C/ebp (L-065627-00-0005) and ATF4 (human, L-005125-00-0020)