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A six-minute gradient elution program was optimized as follows: Initial conditions (10% A, 90% B) transitioned linearly to 50% A at 1.5 min, rapidly increased to 95% A at 1.6 min (held until 4.5 min), and returned to initial conditions at 4.6 min for re-equilibration

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Especficamente, segn la presente descripcin, se proporcionan bencilideno 2,4-pentanodionas segn la frmula I siguiente:Specifically, according to the present disclosure, benzylidene 2,4-pentanediones are provided according to the following formula I: en donde R1 y R2, que pueden ser iguales o diferentes, son independientemente H, alquilo o alcoxi, preferiblemente H, alquilo o alcoxi, en donde los grupos alquilo o alcoxi son lineales o ramificados y tienen de 1 a 8 tomos de carbono, preferiblemente de 1 a 6 tomos de carbono, lo ms preferiblemente de 1 a 4 tomos de carbono y en donde Me es ms preferiblemente metilo, pero tambin puede ser un alquilo inferior lineal o ramificado de 2 a 8 tomos de carbono