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10.2147/OPTO.S321716 13

Peripheral-blood exome, genome, and Sanger sequencing to identify pathologic mutations in SOD1 in 4000 patients with ALS from Germany, South Korea, and Sweden has revealed four sporadic ALS cases with de novo mutations in SOD1 , which might be the therapeutic target of ASO [170]

The MTT solution (0.5 mg/mL) was incubated with cells for 4 h at 37 C and then replaced with 100 L of DMSO to dissolve the formazan product

Activation of macrophages results in the redistribution of HMGB1 from the nucleus to cytoplasm, followed by the release via an unconventional protein secretion pathway (25, 27, 28)