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Ferroptosis markers were evaluated using colorimetric Fe 2+ detection, flow cytometric apoptosis analysis, DCFH-DA probes for reactive oxygen species (ROS), DTNB colorimetry for glutathione (GSH) levels, and western blot (WB) for SLC7A11 and GPX4 expression

Beyond these, I/R injury can also develop in situations of trauma, shock, or sepsis, where systemic disruptions in blood flow to vital organs can precipitate tissue injury