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X100-500ML, Sigma-Aldrich), 10% Horse Serum (26050-070, Gibco) in phosphate buffer) with the primary antibodies (Iba1 (AB48004, Abcam), GFAP (Z0334, Dako), rhodopsin (MAB5316, Merck Millipore), cone arrestin (AB15282, Merck Millipore) for three days at room temperature

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