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First, the presence of ROS may be a primary driving factor of oxidative stress in the urinary system

Esposito, F., et al

The assay is composed of two parts: the preparation of cell cytosolic/tissue extracts and the detection of total glutathione (GSH and GSSG)

Multiple enteroid cultures were harvested with Culturex Organoid Harvesting Solution (Trevigen, United States), fragmented and re-suspended in expansion media and seeded (100 l) on the inner surface of 0.4 m Transwell inserts (Corning, United States) pre-coated with human collagen IV (Sigma-Aldrich, United States)