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On dark incubation the absorbance was read at 390 nm using 30% (v/v) aqueous H 2 O 2 and the concentration as determined using the molar extinction coefficient (1.75 10 5 Lmol 1 cm 1 ) according to [39]

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[DOI] [PMC free article] [PubMed] [Google Scholar] 13.Dougherty PP, Klein-Schwartz W

In addition, established research has shown the suppression in 7nAChR resulting in massive brain oxidative stress with high levels of oxidative markers, including malondialdehyde (MDA) and nitric oxide (NO) with decreased antioxidant enzyme activity, including glutathione peroxidase (GPx) and glutathione (GSH), and all these findings result in developing ASD [36, 62, 70, 71]