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however, certain reports have concluded that PCs are not the primary cause of the hyperaccumulation of Zn, Ni, or Pb (Hossain et al., 2012)

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Sections were then stained with haematoxylin-eosin, or immunostained with antibodies to Aminopeptidase A (ab36122, Abcam, 1:150), MGST2 (Atlas Antibodies HPA010707, 1:50), cleaved caspase-3 (Asp 175 , Cell Signaling #9661, 1:300), NOX 4 (Abcam ab109225, 1:100) or 8-OHdG (QED Bioscience 12501, 1:200)