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Spraying of sulfur solution and serine and glutamine were effective in reducing the negative effects of lead stress, as it reduced the amount of hydrogen peroxide and grain protein and increased the reservoir of glutathione

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After 3 days in EM, the medium was replaced with differentiation medium (DM) containing advanced DMEM/F12, 1% P/S, 1 mM GlutaMax, 1 mM HEPES, 1% N-2, 2% B-27 with vitamin A, 25 ng/mL HGF, 0.5 M A83-01, 100 ng/mL FGF19 (PeproTech), 100 ng/mL BMP7, 1mM N-acetyl L-cysteine, 10 nM Leu15-gastrin I, and 3 M dexamethasone